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Beating can also be heard between notes that are near to, but not exactly, a harmonic interval, due to some harmonic of the first note beating with a harmonic of the second note.
For example, in the case of perfect fifth, the third harmonic (i.e.
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When tuning instruments that can produce sustained tones, beats can be readily recognized.
second overtone) of the bass note beats with the second harmonic (first overtone) of the other note.
As well as with out-of tune notes, this can also happen with some correctly tuned equal temperament intervals, because of the differences between them and the corresponding just intonation intervals: see Harmonic series (music)#Harmonics and tuning.
To experience the binaural beats perception, it is best to listen to this file with headphones on moderate to weak volume – the sound should be easily heard, but not loud.
Note that the sound appears to pulsate only when heard through both earphones.